Latency to veratridine-mediated (100.0 μM) depolarization in EAE and naive mice Spinal cords were removed from SJL/PLP-inoculated EAE and sham (age-matched) mice at between 2 and 5 weeks PI and placed in a grease-gap bath. The d.c. potential between the two compartments was assessed in response to local application of veratridine (10.0, 30.0, and 100.0 μM). The data are represented as the mean ± S.E.M. of the group for the latency to reach the maximum level of depolarization from the point of application of the 100.0 μM concentration of veratridine. Induction of EAE did not affect the latency to maximal depolarization.
Drug Application | Weeks PI | Avg. Sec. (±S.E.M.) | n | EAE Deficit Score Avg. Score (±S.E.M.) | ||||
|---|---|---|---|---|---|---|---|---|
| Region | Group | |||||||
| A. Veratridine (100.0 μM) latency to peak in EAE and sham mice | ||||||||
| Lumbar | Sham | 2 weeks PI | 501 (±31) | 20 | 0 | |||
| 3 weeks PI | 519 (±23) | 19 | 0 | |||||
| 4 weeks PI | 464 (±35) | 18 | 0 | |||||
| 5 weeks PI | 436 (±25) | 19 | 0 | |||||
| Lumbar | EAE | 2 weeks PI | 411 (±30) | 21 | 2.6 (±0.2) | |||
| 3 weeks PI | 570 (±53) | 8 | 2.2 (±0.4) | |||||
| 4 weeks PI | 532 (±52) | 16 | 2.4 (±0.3) | |||||
| 5 weeks PI | 526 (±42) | 11 | 1.1 (±0.3) | |||||
| B. Veratridine (100.0 μM) latency to peak in EAE and sham mice | ||||||||
| Sacral | Sham | 2 weeks PI | 449 (±29) | 20 | 0 | |||
| 3 weeks PI | 453 (±18) | 19 | 0 | |||||
| 4 weeks PI | 478 (±28) | 20 | 0 | |||||
| 5 weeks PI | 451 (±16) | 19 | 0 | |||||
| Sacral | EAE | 2 weeks PI | 458 (±32) | 16 | 2.5 (±0.2) | |||
| 3 weeks PI | 507 (±26) | 7 | 2.3 (±0.5) | |||||
| 4 weeks PI | 428 (±17) | 17 | 2.4 (±0.2) | |||||
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| 532 (±41) | 13 | 1.2 (±0.3) | |||