ED50 values and 95% confidence limits (CL) for rate-decreasing effects of GHB, baclofen, and diazepam alone, and following a dose of antagonist
Test Compound | Dose (mg/kg) Antagonist | n | ED50 | 95% CL | ||
|---|---|---|---|---|---|---|
| GHB | 0 | 8 | 218.6* | 190.2 | 251.2 | |
| CGP 35348 (56) | 8 | 259.0 | 193.3 | 347.1 | ||
| CGP 35348 (100) | 5 | 188.0 | 127.5 | 277.2 | ||
| CGP 35348 (178) | 8 | 302.8 | 240.8 | 380.8 | ||
| GHB | NCS-382 (10) | 6 | 195.4 | 146.2 | 261.1 | |
| NCS-382 (32) | 6 | 188.0 | 136.4 | 259.2 | ||
| GHB | Flumazenil (5.6) | 6 | 234.5 | 170.1 | 323.2 | |
| Baclofen | 0 | 8 | 2.2† | 1.9 | 2.6 | |
| CGP 35348 (56) | 7 | 3.5a | 3.1 | 3.8 | ||
| CGP 35348 (100) | 6 | 3.8a | 2.4 | 6.0 | ||
| CGP 35348 (178) | 7 | 4.5a | 3.9 | 5.1 | ||
| Baclofen | NCS-382 (10) | 6 | 2.4 | 1.9 | 3.0 | |
| NCS-382 (32) | 7 | 3.0 | 2.5 | 3.6 | ||
| NCS-382 (56) | 4 | 2.8 | 1.1 | 6.8 | ||
| Diazepam | 0 | 8 | 2.4 | 1.6 | 3.8 | |
| Flumazenil (1.0) | 6 | 2.9 | 1.4 | 6.4 | ||
| Flumazenil (3.2) | 4 | 5.4b | 3.9 | 7.4 | ||
| Flumazenil (5.6) | 8 | 8.8b | 4.5 | 17.2 | ||
| Diazepam | NCS-382 (10) | 6 | 0.6b | 0.2 | 1.6 | |
| NCS-382 (32) | 7 | 1.7 | 0.9 | 3.3 | ||
|
| NCS-382 (56) | 6 | 2.0 | 0.9 | 4.8 | |
↵* Individual ED50 values were averaged across four determinations; individual average ED50 values were used to calculate the reported ED50 and 95% CL values
↵† Individual ED50 values were averaged across 2 determinations; individual average ED50 values were used to calculate the reported ED50 and 95% CL values
↵ a Significant difference (α = 0.05) from control baclofen ED50 value (Dunnett's test)
↵ b Significant difference (α = 0.05) from control diazepam ED50 value (Dunnett's test)