Primers used for amplification of CYP2B6 cDNA, CYP2B6 exons, and 5′-UTR in genomic DNA
Designation | 5′ primer (5′ to 3′) | 3′ primer (5′ to 3′) | Annealing Temperature (°C) | Cycles | Extension Time |
|---|---|---|---|---|---|
| Primers used for amplification of CYP2B6 cDNA | |||||
| CYP2B6/utrF1/R1 | CAGTTGGAGGCTGCAGCA | CCTGCACTCACTTGCAATGT | 57.3 | 32 | 1.45 |
| CYP2B6/utrF2/R2 | CAGACCATGGAACTCAGCGT | ATGACCCTGGAATCCTTTGAC | 57.8 | 18 | 1.30 |
| CYP2B6/2-7F/R | ATGGTCGACCCATTCTTCCG | GGCATTTTGGCTCGGTCATG | 58 | 18 | 1 |
| CYP2B6/8AF/R | AGAGCTTCATGACCGAGCCAA | ATGACCCTGGAATCCTTTGAC | 57.16 | 18 | 1 |
| CYP2B6/3AF/R | CAGTGCTGAGCCTGGTGTAT | CGAATACAGAGCTGATGAGTG | 55.8 | 18 | 1 |
| CYP2B6/3A′F/R | GGTCGACCCATTCTTCCG | ACACCAGGCTCAGCACTG | 55.5 | 18 | 1 |
| Primers used for amplification of CYP2B6 exons and 5′-UTR region in genomic DNA | |||||
| CYP2B6/ex1F1/R1 | GCTAATGCTCCTGGATGATG | CATGCTTCACCATGCTCC | 56.18 | 35 | 1 |
| CYP2B6/ex2-3F/R | CTTAATTGCTGGGTCCCA | GTCTTTCTTTCCTATTCATCC | 55.06 | 35 | 1 |
| CYP2B6/ex4F/R | GGTCTGCCCATCTATAAAC | CTGATTCTTCACATGTCTGCG | 54.5 | 35 | 1 |
| CYP2B6/ex5F/R | AGAAAGACAAATAAACAGGCT | CTTTCTTTCCTTCCTCATGTT | 55.26 | 35 | 1 |
| CYP2B6/ex6F/R | TAGAGATACGCGGTTGGATG | TGGCTTGGTTGGAATCTAGC | 56.9 | 35 | 1 |
| CYP2B6/ex7F/R | AGGAATCCACCCACCTCAAC | CAACCCTCCACACACTCCA | 56.78 | 35 | 1 |
| CYP2B6/ex8F/R | TAGCTCCTTAATTGAGTCCCG | GATAAGGCAGGTGAAGCAATCA | 56.6 | 35 | 1 |
| CYP2B6/ex9F/R | GCATCTGGTTTCAGAGCAGC | CCTGCACTCACTTGCAATGT | 56.7 | 35 | 1 |
| CYP2B6/3AintF/R | TGCCGTGAGCTAAGATCACA | GTAGTCCCAGTTGAAATAGTCC | 55.6 | 35 | 1 |
| CYP2B6/8AintF/R | TAGCTCCTTAATTGAGTCCCG | TCCTGCTTGCCAAGATGTG | 56.78 | 35 | 1 |
| CYP2B6/5′UTRF/R | CTCAGGCTGGCCGACACT | GGTCATGGGTGTTAGGGTGG | 57.5 | 35 | 2.15 |
| CYP2B6/XREMF1/R1 | ATTAGGCCATACTTGTGTTGC | TTGATAGACACTTAGGTTGATTG | 53 | 35 | 1 |
| CYP2B6/XREMF2/R2 | ATTCTGCTGAGAGAGGCATC | ATGTGTCCACATCTGCTGTC | 56 | 35 | 1 |
| Additional sequencing primers | |||||
| CYP2B6/5′UTRF1/R1 | GCAAGGATACACACATAAGCAC | GGTCATGGGTGTTAGGGTGG | |||
| CYP2B6/5′UTRF2/R2 | GAGATGGAGTATTGCTGTGTC | TGCTTGCATCCCTGTATGTG | |||
| CYP2B6/5′UTRF3/R3 | ACACACCTGGAGCTCAAGAG | CAGTGAGCCAAGATTACACC | |||
| CYP2B6/5′UTRF4/R4 | CCCAACCCACTGTATGACCT | GGAGTTTCTTCCCTGCCTCT | |||
| CYP2B6/5′UTRF5/R5 | CTCAGGCTGGCCGACACT | AGTGAGGTATTGTTGCCATCC |
|
|
|
UTR, untranslated region.