TABLE 6

Kinetics of morphine glucuronidation in microsomal preparations obtained from the liver of rats treated with heroin or cadmium chloride given alone or in combination

Data are expressed as mean ± S.E.M.

	M3G
	K_m	V_max	V_max/K_m	K_m	V_max
	mM	nmol/min/mg		mM	pmol/min/mg
Saline	0.2 ± 0.01	8.1 ± 0.1	39.1 ± 0.9	n.d.	n.d.
Repeated cadmium (15 μg/kg × 10)	0.2 ± 0.01	5.7 ± 0.05†	29.2 ± 1.8†	n.d.	n.d.
Repeated heroin (10 mg/kg × 10)	0.2 ± 0.1	7.2 ± 0.04†^*‡	33.8 ± 1.2	33.5 ± 0.2	36.4 ± 0.5
Repeated heroin and cadmium (10 mg/kg + 15 μg/kg × 10)	0.2 ± 0.1	6.0 ± 0.01†‡	33.6 ± 1.0	31.5 ± 0.6	34.6 ± 0.5

n.d., not detectable.
↵* P < 0.05 versus repeated heroin + cadmium (Tukey's test).
↵† P < 0.05 versus saline (Tukey's test).
↵‡ P < 0.05 versus cadmium (Tukey's test).