Agenta | Specific NT Binding (% Control) at Dose of Agentb | ||||||
---|---|---|---|---|---|---|---|
10 μM | 25 μM | 75 μM | 100 μM | ||||
NIF | 97 ± 5 | 102 ± 5 | 108 ± 8 | ||||
Nimodipine | 99 ± 2 | 101 ± 2 | 94 ± 4 | ||||
Phloretin | 110 ± 7 | 105 ± 8 | 97 ± 8 | ||||
Verapamil | 100 ± 2 |
| 101 ± 5 | 103 ± 4 |
↵ a Agents were freshly dissolved in DMSO at 10 mM and diluted into binding buffer just before use.
↵ b PC3 cell membranes were preincubated 10 min with agents or control, and NT binding was performed at 22°C for 60 min (see under Materials and Methods). Specific binding was measured in four to six experiments and expressed as percentage of control (mean ± S.E.M.). Results for the various agents were not significantly different from control (p > 0.1).