Amplicon Coordinatesa | Primer Sequence (5′ to 3′) | Exon | PCR Conditionsb | ||
---|---|---|---|---|---|
Forward
|
Reverse
| ||||
−10,443 to −10,003 | CTTTTGGTTTTCCTGGTATG | GGAGTCTGATCTTGGGAAT | A | ||
−10,078 to −9,573 | TTCCAACATGACTCTGATAA | GCACAGTGGATAAACCTG | A | ||
−9,647 to −9,413 | AAATATAAACAACGGCTAACCT | AGACCCCACTTTCCTCAT | 1 | B | |
−137 to +153 | GAGACCCGCTACAGAAGAT | AGTTGTTGAAACCCCACTT | 2 | C | |
+9,442 to +9,669 | CACTGTTTGTACAGGAAC | AGAGAAAATAGGTAGGAAAT | 3 | C | |
+17,224 to +17,521 | ACAACTGCTAATCATATCTG | CTATTTAATGGAACAGCC | 4 | C | |
+20,600 to +20,808 | TCCTGTTAATTCTCTGTGTGAC | CACCCAGTAACATCAGGAA | 5 | C | |
+22,651 to +22,935 | AGCCGTGTTCACAGTGTCA | TCTTCCTAAAAGCCCTTGG | 6 | C | |
+23,856 to +24,293 | TGACAAGTCTCCTCCCTGTT | TCACATGGCACACCAAA | 7 | B | |
+24,943 to +25,153 | TGAGTAGCTGGTACTAGACGTG | GGGTGCATTAAGTAGTTAACTTACC | 8 | B | |
+27,093 to +27,449 | CCCCAATCTTCCTTTTATA | AAATCAGAAAAATAGCCACA | 9 | B | |
+27,380 to +27,648 | CCATCTCCCTTTGAAAGTT | TCTAGCATTAGAAGTGGAGC | 9 | A | |
+27,595 to +27,928
|
CCATCTCCCTTTGAAAGTT
|
TCTAGCATTAGAAGTGGAGC
|
9
|
A
|
↵ a Coordinates are based on assigning the “A” of the ATG translation initiation codon +1.
↵ b A, denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 92°C for 30 s, annealing at 50°C for 30 s, and elongation at 75°C for 90 s, and a final elongation at 75°C for 5 min; B, denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 92°C for 30 s, annealing at 55°C for 30 s, and elongation at 75°C for 90 s, and a final elongation at 75°C for 5 min; C, denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 92°C for 30 s, annealing and elongation at 55°C for 90 s, and a final elongation at 75°C for 5 min.