TABLE 3

Effect of avasimibe and prototypical inducers on CYP3A4 and MDR1 mRNA in primary human hepatocytes

Results are expressed as fold increase over control hepatocytes. Primary human hepatocytes (n = 3 wells/treatment) were incubated for 48 h with 0.1% DMSO control or one of the positive controls: rifampin or phenobarbital or negative controls β-NF or 3-MC or avasimibe at 1 or 5 μg/ml. At the end of these treatments, the culture medium was discarded; RNA extracted, reverse transcribed, and fluorescently labeled; and microarray analysis was conducted as described under Materials and Methods. Values represent fold increase over control. Each sample was analyzed in quadruplicate and each value represents the mean ± S.D. of two preparations.