TABLE 3

Effect of protease inhibitors on VIP_4-28 in Molt-4b lymphoblasts

Inhibitor concentrations were bacitracin (1 mg/ml), PMSF (0.5 mM), DFP (5 mM), PA (5 μM), β-thiorphan (β-T) (4 μg/ml), bestatin (1 mM) and pepstatin (1 mM). Molt-4b lymphoblasts (1 × 10⁶ cells/point) were incubated for 5 min at 37°C in the presence of indicated protease inhibitor and adenylate cyclase agonist. Reaction was stopped by the addition of TCA, and cAMP was quantified by radioimmunoassay as described under Materials and Methods.