Relative expression of mRNA of Mac-1, TLR-4 (markers for microgliosis), and GFAP (marker for astrogliosis) in the sham-operated and L5 lumbar spinal cord of L5 nerve-transected rats (saline or minocycline treated)
The mRNA levels were normalized to the corresponding GAPDH (housekeeping gene) level for each sample to adjust for uncontrolled variability between samples. Values are mean ± S.E.M. (n = 4).
Sham | Saline | Minocycline (40 mg/kg) Treatment | |||
|---|---|---|---|---|---|
| Preemptive | Postinjury | ||||
| Mac-1 | 2.9 ± 0.3 | 12.6 ± 0.5a | 3.6 ± 0.6a,b | 3.9 ± 0.5a,b | |
| TLR-4 | 0.4 ± 0.02 | 1.4 ± 0.05a | 0.6 ± 0.03a,b | 0.7 ± 0.04a,b | |
|
GFAP
| 111.2 ± 3.7 | 290.6 ± 12.8a | 147 ± 7.9a,c | 278.5 ± 14.1a | |
↵ a p < 0.01 vs. sham; bp < 0.01; and cp < 0.05 vs. saline-treated group.