Primers and Taqman probe sequence for the rat genes characterized in this experiment
Genes | Accession No. | Primers/Probes | Primers and Probesa |
|---|---|---|---|
| Forward primer | 5′-CCCCCAATGTATCCGTTGTG-3′ | ||
| GAPDH | NM_01008 | Reverse primer | 5′-TAGCCCAGGATGCCCTTTAGT-3′ |
| Taqman probe | 5′-TGCCGCCTGGAGAAACCTGCC-3′ | ||
| Forward primer | 5′-CTGCCTCAGGGATCCGTAAAG-3′ | ||
| Mac-1 | U59801 | Reverse primer | 5′-CCTCTGCCTCAGGAATGACATC-3′ |
| Taqman probe | 5′-CCCGGGACAATGCCGCGAA-3′ | ||
| Forward primer | 5′-GATTGCTCAGACATGGCAGTTTC-3′ | ||
| TLR4 | NM_0191178 | Reverse primer | 5′-CACTCGAGGTAGGTGTTTCTGCTAA-3′ |
| Taqman probe | 5′-TCCTTGCTGAGGCAGCAGGTCGAAT-3′ | ||
| Forward primer | 5′-TGGCCACCAGTAACATGCAA-3′ | ||
| GFAP | NM_017009 | Reverse primer | 5′-CAGTTGGCGGCGATAGTCAT-3′ |
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| Taqman probe | 5′-CAGACGTTGCTTCCCGCAACGC-3′ |
↵ a The Taqman probe has a reporter fluorescent dye, FAM (6-carboxyfluorescein) at the 5′ end and fluorescence dye quencher, TAMRA (6-carboxytetramethyl-rhodamine) at the 3′ end.