TABLE 3

Binding affinities (Ki) of various ligands on membranes of CHO cells transfected with the hPrRP receptor

The affinities of the ligands were determined in competition binding assays with [125I]PrRP20, as described under Materials and Methods. Data are given as the average of two experiments or as mean ± S.E.M. if three or more experiments were conducted. If the displacement curve was incomplete, the Ki is given as greater than the highest concentration tested.
Ligand
Ki
nM
NPFF >100,000
hPrRP31 0.68 ± 0.06
hPrRP20 1.0 ± 0.3
hPrRP24-31 47 ± 11
hRFRP-1 >100,000
hRFRP-3 >50,000
BIBP3226 13,000 ± 2,000
hNPY18-36
7,400