Unidirectional and net Cl- fluxes (J) and apparent permeability coefficient (Papp) of D-mannitol across the control and Ad5 infected conjunctival tissues in the presence and absence of 10 μM UTP added mucosally
Each data entry represents mean ± S.E.M. Numbers in parentheses are the number of tissues used. Transport of 36Cl was studied at 0.5 μCi/ml and D-[14C] mannitol at 10 μCi/ml. Jnet equals Jsm - Jms, where J is the chloride flux, sm and ms denote serosal-to-mucosal and mucosal-to-serosal directions, respectively. A positive value for Jnet represents secretion and a negative value absorption.
Ad5 | UTP | Cl- Flux (μEq/(h · cm2)) | D-Mannitol Papp (×10-7 cm/s) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Jsm | Jms | Jnet | Jsm | Jms | Jnet | ||||||
| - | - | 0.59 ± 0.05 (10) | 0.34 ± 0.05 (7) | 0.25 ± 0.06 | 1.82 ± 0.20 (10) | 2.1 ± 0.29 (7) | -0.28 + 0.11 | ||||
| + | 0.97 ± 0.09 (10) | 0.42 ± 0.10 (7) | 0.55 ± 0.13a | 2.01 ± 0.19 (10) | 1.99 ± 0.30 (7) | 0.02 ± 0.36 | |||||
| + | - | 0.36 ± 0.10 (5) | 0.25 ± 0.08 (4) | 0.11 ± 0.10a,b | 3.2 ± 0.89 (5) | 2.33 ± 0.21 (4) | 0.87 ± 0.91 | ||||
|
| + | 0.64 ± 0.07 (5) | 0.31 ± 0.18 (4) | 0.33 ± 0.19b | 2.49 ± 0.33 (5) | 2.25 ± 0.41 (4) | 0.24 ± 0.53 | ||||