CYP3A9 | Upstream Primer Downstream Primer Fluorogenic Probe | 5′ GGTGTTGTATCACATGGACCAGA 3′ 5′ CCAGGAGTGAACAAAATTACTGCA 3′ 5′Fam-CCAGTTCATATTGTGAATGAATCCAGAGATGAAGA-Tamra 3′ |
Rat cyclophilin | Upstream Primer Downstream Primer Fluorogenic Probe | 5′ CGATGACGAGCCCTTGG 3′ 5′ TCTGCTGTCTTTGGAACTTTGTC 3′ 5′Fam-CGCGTCTCCTTCGAGCTGTTTGCA-Tamra 3′ |
Primers and probes were designed using Primer Express software (Applied Biosystems) at the 3′-untranslated region of the CYP3A9 gene since this region is highly differentiable among all CYP3A isoforms. Similarly,R-cyclophilin primers and probe were also designed using Primer Express software.