[3H]Rolipram | [3H]Piclamilast | |||||
---|---|---|---|---|---|---|
Whole | Membrane | Cytosolic | Whole | Membrane | Cytosolic | |
B max (fmol/mg protein) | 273.3 ± 31.81-a | 280.0 ± 16.5 | 210.0 ± 20.0 | 430.0 ± 28.9 | 453.0 ± 18.6 | 373.3 ± 63.3 |
K d(nM) | 19.2 ± 10.11-a | 4.8 ± 0.5 | 12.1 ± 2.2* | 6.7 ± 2.3 | 3.1 ± 0.2 | 4.5 ± 1.1 |
%HARBS | 1001-a | 100 | 100 | 62.1 ± 1.0 | 58.7 ± 4.2 | 63.3 ± 9.2 |
↵1-a Values shown are Bmax andKd values and percentage of binding to the HARBS (mean ± S.E.M.) from at least three independent experiments, determined by nonlinear regression (Draper and Smith, 1966; O'Donnell et al., 1984).
1-150 Significantly different from corresponding value obtained using membrane preparations, p < 0.05.