Characterization of spinal transmission in several nociception tests
| Test | Vehicle | CP-99994 | MK-801 | CNQX |
|---|---|---|---|---|
| Tail-flick (s) | 10.4 ± 0.5 | 13.5 ± 1.4 | 35.7 ± 4.0* | 33.4 ± 4.3* |
| Hargreaves (s) | 8.7 ± 0.4 | 13.2 ± 0.9* | 12.6 ± 1.3* | 9.8 ± 0.9 |
| Paw pressure (g) | 10.4 ± 0.7 | 10.3 ± 0.3 | 15.6 ± 0.6* | 11.0 ± 0.8 |
| Capsaicin (s) | 60.1 ± 3.0 | 29.2 ± 2.0* | 30.9 ± 4.5* | 60.0 ± 1.1 |
| ANF | ||||
| BK (2 pmol)1-a | 68.9 ± 8.4 | 14.5 ± 5.6* | 70.1 ± 7.1 | 57.7 ± 6.5 |
| SP (10 pmol) | 61.9 ± 11.6 | 6.7 ± 3.3* | 69.0 ± 8.3 | 54.0 ± 5.9 |
| ATP (100 pmol) | 63.3 ± 6.9 | 66.2 ± 5.2 | 24.9 ± 6.8* | 51.7 ± 8.1 |
| PGI2(100 pmol)1-a | 60.6 ± 8.9 | 68.3 ± 5.4 | 19.8 ± 9.3* | 49.3 ± 6.7 |
Various antagonists (3 nmol) were given intrathecally 20 min before tail-flick, paw pressure, Hargreaves, or capsaicin test. In ANF test, various antagonists (100 pmol) were given 20 min before bradykinin (BK), substance P (SP), ATP, or prostagrandin I2 agonist (PGI2) stimulation.
1-150 P < 0.05, compared with vehicle-treated mice.
↵1-a These data are quoted from our previous report (Ueda et al., 2000b) to compare with other nociception tests.