Effect of different concentrations of BP on DNA adduct levels
| Adduct | BP (0.03 μM) | BP (0.3 μM) | BP (3 μM) |
|---|---|---|---|
| 1 | <0.02 | 3.69 ± 0.1 | N.D. |
| 2 | 0.62 ± 0.1 | 63.70 ± 7.6 | 149.63 ± 22.8 |
| 3 | 3.16 ± 0.5 | 28.52 ± 3.4 | 13.16 ± 2.4 |
| 4 | <0.02 | 0.85 ± 0.1 | 1.68 ± 0.4 |
| 5 | <0.02 | 1.51 ± 0.3 | 2.57 ± 0.5 |
| 6 | <0.02 | 2.58 ± 0.3 | 1.41 ± 0.2 |
| 7 | <0.02 | 0.30 ± 0.04 | 0.45 ± 0.1 |
| 8 | <0.02 | 0.16 ± 0.02 | 0.39 ± 0.02 |
| Total | 3.78 ± 0.5 | 101.31 ± 9.4 | 169.9 ± 28.4 |
Adduct values represent the means ± S.D. of three independent experiments and are expressed as RAL × 108. BP treatment of cells, DNA isolation, and 32P-postlabeling were carried out as described under Materials and Methods. The limit of detection, expressed as RAL × 108, was 0.02.
N.D., not determined.