Table 2
Inhibitory potencies of entacapone and tolcapone against rat liver total COMT
| Entacapone | Tolcapone | |
|---|---|---|
| nM | ||
| IC50 | 20.1 (15.7–25.9) | 19.7 (15.8–24.4) |
| K i app | 27.3 (19.3–35.3) | 25.4 (19.2–31.6) |
| K i | 10.7 (7.6–13.9) | 10.0 (7.6–12.4) |
IC50 and Kiapp values are best least-squares fit values calculated from six independent experiments. The 95% confidence intervals are given in parentheses.Ki values were calculated fromK
values by the equationKiapp=Ki(1+[S]/Km) , where S is the substrate concentration.