Table 5

Oxidation of 7-butoxycoumarin by 2B1dH, 2B6dH, 2E1dH, 2B6dH, and 2E1dH mutants5-a

P4507-HC5-b4-OH5-b3-OH5-b2-OH5-bTotal Activity5-b
2B1dH3.8 (29)5-c 0.7 (5)5.5  (43)2.9 (23)13
2B6dH0.2 (1)1.1 (9)10  (83)0.9 (7)12
2E1dH0.1 (2)0.6 (10)5.2  (88)N.D.5.9
2B6dH I209LN.D.0.5 (7)6.2  (88)0.3 (5)7.0
2B6dH S294DN.D.0.4 (12)2.5  (88)N.D.2.8
2B6dH L363V0.7 (19)0.2 (5)1.9  (50)1.0 (26)3.9
2B6dH V367LN.D.1.4 (12)9.6  (80)1.0 (8)12
2B6dH V477F0.4 (1)2.3 (7)27  (88)1.3 (4)31
2E1dH A103MN.D.0.1 (8)0.8  (92)N.D.0.9
2E1dH L209IN.D.0.5 (15)2.8  (85)N.D.3.4
2E1dH V363LN.D.2.7 (47)3.0  (53)N.D.5.7
2E1dH L367VN.D.0.3 (9)3.3  (91)N.D.3.6
2E1dH F477VN.D.0.3 (8)3.3  (92)N.D.3.6
  • N.D., not detected.

  • 5-a The concentration of 7-butoxycoumarin utilized was 300 mM.

  • 5-b Values are expressed as nmol/min/nmol of P450.and are derived from duplicate incubations performed as described under Materials and Methods.

  • 5-c Values in parentheses indicate percentages of total activity.