Table 3

Kinetic parameters of 7-EC-deethylation, 7-EFC deethylation, and pNP hydroxylation

P4507-EC7-EFCpNP
S 50 3-a n V max 3-b K m 3-a V max 3-b K m 3-a V max 3-b
2B6dH911.43.68.514N.D.3-c N.D.3-c
2E1dH461.63.0N.D.3-c N.D.3-c 7033

Values are the means of two independent experiments performed in duplicate and conducted as described under Materials and Methods.

  • ↵3-a Km,S50, μM.

  • ↵3-b Vmax, nmol/min/nmol of P450.

  • ↵3-c Because of very low activity, the kinetic parameters could not be determined (N.D.).