Specific S-(−)-[3H]nicotine binding affinity (Kd) and maximum densities (Bmax) in the absence and presence of theN-n-alkylnicotinium analogs NDNI and NONI
| Analog | Concentration | Kd | 95% CI3-a | Bmax | S.E.M. | Simple- vs. Variable-Slope Sigmoid Fit F-Statistic |
|---|---|---|---|---|---|---|
| μ M | nM | fmol/mg protein | ||||
| NDNI | 0 | 2.15 | 156, 2.75 | 69.1 | 3.4 | F 1,5 = 2.64,P = 0.16 |
| 0.6 | 3.96 | 3.19, 4.72 | 79.5 | 3.4 | F 1,7 = 0.76, P= 0.41 | |
| 2 | 5.69 | 4.77, 6.62 | 87.3 | 3.5 | F 1,7 = 2.33, P= 0.17 | |
| 6 | 13.4 | 9.16, 17.5 | 88.5 | 8.6 | F 1,6 = 0.18, P= 0.68 | |
| NONI | 0 | 1.17 | 0.89, 1.45 | 62.2 | 1.9 | F 1,7 = 1.45, P= 0.27 |
| 0.66 | 2.55 | 2.12, 2.97 | 64.9 | 2.0 | F 1,7 = 5.30, P= 0.05 | |
| 2.2 | 4.27 | 3.48, 5.07 | 64.0 | 2.8 | F 1,7 = 1.26, P= 0.30 | |
| 22 | 17.6 | 10.6, 24.6 | 57.3 | 7.5 | F 1,8 = 0.08, P= 0.78 |
Parameters were determined from studies using rat striatal membranes.Kd and Bmax values were derived from linear regression analysis of the Scatchard-transformed data from saturation binding isotherms. F-statistics were calculated for comparison of simple- vs. variable-slope sigmoid curve fits.
↵3-a 95% CI = 95% confidence interval.