Table 1

Inhibition of specific S-(−)-[³H]nicotine binding to rat striatal membranes by novelN-n-alkylnicotinium analogs: comparison with reference compounds DHβE, S-(−)-nicotine, andR-(⁺)-nicotine

Compound	n-Alkyl Chain Length	IC₅₀	95% CI^1-a	K_i	95% CI	Slope Factor^1-b	s.e.^1-c
		μM		μM
NDNI	10	0.23	0.20, 0.27	0.09	0.08, 0.11	−0.958	0.066
NDDNI	12	0.35	0.28, 0.43	0.14	0.11, 0.17	−0.854	0.053
NHxNI	6	1.32	1.15, 1.50	0.53	0.46, 0.60	−0.904	0.045
NNNI	9	2.09	1.83, 2.39	0.84	0.73, 0.95	−1.064	0.045
NENI	2	2.60	1.89, 3.58	1.04	0.76, 1.43	−1.106	0.179
NHpNI	7	5.14	4.64, 5.70	2.06	1.85, 2.28	−1.031	0.027
NMNI	1	8.82	6.94, 11.2	3.53	2.77, 4.48	−0.957	0.047
NnBNI	4	27.2	24.2, 30.7	10.9	9.70, 12.3	−1.107	0.089
NPNI	3	53.7	45.6, 63.4	21.5	18.2, 25.3	−0.936	0.076
NONI	8	49.3	38.6, 62.9	19.7	15.4, 25.1	−1.136	0.112
DHβE	NA^1-d	0.36	0.24, 0.53	0.14	0.09, 0.21	−0.667	0.067
S-(−)-Nicotine	0	0.002	0.001, 0.004	0.0008	0.0007, 0.0040	−0.816	0.029
R-(⁺)-Nicotine	0	0.12	0.10, 0.15	0.05	0.04, 0.06	−0.993	0.091

IC₅₀ values were derived from variable-slope, sigmoid curve fits to the mean data from four to six independent observations.K_i values were derived from IC₅₀values using the Cheng-Prusoff equation.

↵1-a 95% CI = 95% confidence interval.
↵1-b Pseudo-Hill coefficient, derived using a variable slope model.
↵1-c s.e. = standard error of slope factor.
↵1-d NA = not applicable.