Genotype | Treatment | Time | Pixel Density Liver CYP1A1/1A2 | Pixel Density Lung CYP1A1 |
---|---|---|---|---|
days | ||||
WT | CO | 1 | 80 ± 20 | 190 ± 40 |
WT | MC | 1 | 1275 ± 1801-a | 3243 ± 3501-a |
KO | CO | 1 | N.D. | 100 ± 32 |
KO | MC | 1 | 956 ± 110 | 3996 ± 3201-a |
WT | CO | 8 | 92 ± 10 | 390 ± 36 |
WT | MC | 8 | 825 ± 1501-a | 3898 ± 4081-a |
KO | CO | 8 | N.D. | 360 ± 40 |
KO | MC | 8 | 775 ± 80 | 3386 ± 3181-a |
WT | CO | 15 | 96 ± 12 | 402 ± 44 |
WT | MC | 15 | 900 ± 1001-a | 3980 ± 4121-a |
KO | CO | 15 | N.D. | 440 ± 60 |
KO | MC | 15 | 474 ± 80 | 4164 ± 5601-a |
WT or CYP1A2-null (KO) mice were treated with vehicle CO or MC, once daily for 4 days, and were sacrificed at 1, 8, or 15 days after the last time point. Liver or lung microsomes were isolated from individual animals and CYP1A1/1A2 apoprotein expression was determined by Western blotting, as described under Material and Methods. Band intensities of CYP1A1/1A2 proteins were estimated by densitometry of the Western blots. Data represent means ± S.E. (n= 3).
N.D., not detectable; KO, knockout.
↵1-a Statistically significant differences between vehicle and MC-treated mice at P < 0.05.