Table 1

Brain homogenate levels (nanograms per gram wet tissue) of DA, DOPAC, HVA, noradrenaline (NA), DHPG, and MHPG of both sexes of GBR 12909-treated (20 mg/kg, i.p.) Comt gene-disrupted mice

	Male		Female
	WT	HET	HOM	WT	HET	HOM
Striatum
DA	13,160 ± 606	16,051 ± 743^1-a	17,184 ± 736^1-aa	13,745 ± 786	15,886 ± 742	16,795 ± 800
DOPAC	501 ± 30	859 ± 46^1-aa	2,743 ± 119^1-248 ^1-249	460 ± 28	677 ± 48^1-a ^1-b	2,429 ± 72^1-248 ^1-249
HVA	1,840 ± 107	1,527 ± 84	N.D.	1,381 ± 52^1-bb	1,247 ± 75^1-b	N.D.
NA	59.4 ± 5.6	59.6 ± 2.7	77.7 ± 4.7^1-c	58.4 ± 4.0	59.0 ± 4.5	98.8 ± 7.6
DHPG	N.D.	N.D.	158 ± 5	N.D.	N.D.	143 ± 6
MHPG	61.2 ± 1.7	66.8 ± 2.5	N.D.	59.6 ± 2.1	59.8 ± 1.6	N.D.
Cortex
DA	36.8 ± 4.6	51.0 ± 3.7^1-a	52.7 ± 3.4	39.0 ± 2.3	48.4 ± 2.6	48.4 ± 3.6
DOPAC	14.4 ± 4.0	24.3 ± 1.7	130.8 ± 9.4^1-248 ^1-249	11.4 ± 1.0	22.1 ± 2.9	140.7 ± 17.5^1-248 ^1-249
NA	374 ± 21	442 ± 15	408 ± 15	385 ± 14	418 ± 13	332 ± 20^1-cc ^1-b
DHPG	N.D.	N.D.	223 ± 7	N.D.	N.D.	197 ± 11
MHPG	48.5 ± 18.4	47.4 ± 5.8	N.D.	26.3 ± 3.6	40.1 ± 10.8	N.D.
Hypothalamus
DA	330 ± 21	445 ± 37	795 ± 181^1-a	448 ± 42	515 ± 56	637 ± 66
DOPAC	108 ± 8	206 ± 8	792 ± 68^1-248 ^1-249	108 ± 5	197 ± 22	796 ± 51^1-248 ^1-249
HVA	315 ± 13	252 ± 10^1-aa	N.D.	259 ± 12^1-bb	252 ± 25^1-aa	N.D.
NA	1,419 ± 74	1,455 ± 83	1,575 ± 128	1,326 ± 93	1,426 ± 100	1,517 ± 114
DHPG	N.D.	N.D.	482 ± 33	N.D.	N.D.	452 ± 21
MHPG	128 ± 12	125 ± 8	N.D.	100 ± 9	106 ± 15	N.D.

DHPG levels of WT and HET, and cortical HVA levels were under the detection limit.

WT, wild-type; HET, heterozygous; HOM, homozygous; N.D., not detectable.
Statistics:
↵1-a p < 0.05,
↵1-aa p < 0.01, and
↵1-248 p < 0.001 indicate the difference between the corresponding WT groups.
↵1-b p < 0.05 and
↵1-bb p < 0.01 indicate the difference between the corresponding male. HOM marked with
↵1-c p < 0.05,
↵1-cc p < 0.01 and
↵1-249 p < 0.001 were compared with corresponding HET group. Data are means ± S.E.M. of 8 to 11 animals.