In Vitro-Modeled Heterozygotes | CYP2D6*1/*2 | CYP2D6*1/*10 | CYP2D6*1/*17 | CYP2D6*2/*10 | |
---|---|---|---|---|---|
One-enzyme kinetic model fit: | V max (pmol/pmol P450/min) | 7.19 ± 0.40 | 2.70 ± 0.17 | 3.39 ± 0.13 | 4.21 ± 0.21 |
K m (mM) | 3.47 ± 0.78 | 10.8 ± 5.50 | 4.41 ± 0.87 | 23.5 ± 6.33 | |
V max(1) (pmol/pmol P450/min) | 3.57 ± 1.10 | 1.90 ± 0.21 | 2.04 ± 0.87 | 2.38 ± 0.36 | |
Two-enzyme kinetic model fit: | K m(1) (mM) | 0.79 ± 0.52 | 4.22 ± 1.40 | 1.60 ± 1.29 | 5.23 ± 1.94 |
V max(2) (pmol/pmol P450/min) | 5.04 ± 0.87 | 2.69 ± 1.71 | 1.61 ± 0.79 | 2.57 ± 0.29 | |
K m(2)(mM) | 25.4 ± 17.4 | 2050 ± 2730 | 39.0 ± 48.5 | 322 ± 175 |
All reactions were run in duplicate. Error estimates are based on the best fit of the average values obtained at each point to the Michaelis-Menten equation (or a two-enzyme equation) using nonlinear regression analysis.