Recombinant CYP2D6 isoforms: purity and molecular weight information obtained from SDS-polyacrylamide gel electrophoresis analyses and mass spectrometry
| CYP2D6 Allele | 2D6*1 | 2D6*2 | 2D6*10 | 2D6*17 |
|---|---|---|---|---|
| Genomic nucleotide changes: | Ø | G1749C, C2938T, G4268C | C188T, G1749C, G4268C | C1111T, G1749C, C2938T, G4268C |
| Protein differences (nomenclature): | Ø (CYP2D6.1) | Arg296→ Cys, Ser486→ Thr (CYP2D6.2) | Pro34→ Ser, Ser486→ Thr (CYP2D6.10) | Thr107→ Ile, Arg296→ Cys, Ser486→ Thr (CYP2D6.17) |
| Purity | ||||
| From PAGE | >95 | >95 | >75 | >95 |
| From LC | >90 | >90 | >70 | >99 |
| MW (DA) | ||||
| Predicted | 55769.6 | 55730.6 | 55773.6 | 55742.6 |
| Measured | 55772.0 | 55730.5 | 55794.5 | 55736.0 |
| Difference | +2.4 | −0.1 | +20.9 | −6.6 |
| Error (PPM) | 43 | 1.7 | 374.7 | 118.4 |