Competition binding conditions at recombinant, human dopaminereceptors
| Receptor | Tissue | Radioligand (nM) | Buffer (mM) | pH | Nonspecific (μM) | Time, temperature | Kd |
|---|---|---|---|---|---|---|---|
| (nM) | |||||||
| hD1 | L cells | [3H]SCH23390 (0.3) | Tris-HCl (50), NaCl (120), KCl (5), CaCl2 (2), MgCl2(5) | 7.4 | (+)Butaclamol (3) | 60 min, 25°C | 0.53 |
| hD2S | CHO cells | [125I]iodosulpride (0.1) | Tris-HCl (50), NaCl (120), KCl (5), CaCl2 (2), MgCl2(5) | 7.4 | Raclopride (10) | 30 min, 22°C | 0.45 |
| hD2L | CHO cells | [125]iodosulpride (0.1) | Tris-HCl (50), NaCl (120), KCl (5), CaCl2 (2), MgCl2 (5) | 7.4 | Raclopride (10) | 30 min, 22°C | 0.45 |
| hD3 | CHO cells | [125I]iodosulpride (0.2) | Tris-HCl (50), NaCl (120), KCl (5), CaCl2 (2), MgCl2 (5) | 7.4 | Raclopride (10) | 30 min, 22°C | 0.98 |
| hD4 1-a | CHO cells | [3H]spiperone (0.5) | Tris-HCl (50), NaCl (120), KCl (5), MgCl2 (5), EDTA (1) | 7.4 | Haloperidol (1) | 60 min, 22°C | 0.38 |
| hD5 | CH4Cl cells | [3H]SCH23390 (0.3) | Tris-HCl (50), NaCl (120), KCl (5), MgCl2 (5), EDTA (1) | 7.4 | SCH23390 (10) | 60 min, 22°C | 0.25 |
CHO, Chinese hamster ovary.
↵1-a h4.4. isoform.