Effect of ERBF on IL-8-induced chemotaxis
| Treatment | Fluorescence Intensity |
|---|---|
| Nontreatment | 84.3 ± 7.4 |
| IL-8, 20 ng/ml | 2635.3 ± 282.8 |
| ERBF, 20 μM | 122.5 ± 20.8 |
| IL-8, 20 ng/ml + monoclonal antibody, 5 μg | 527.6 ± 50.3 |
| IL-8, 20 ng/ml | |
| + ERBF, 20 μM (top well) | 2869.7 ± 297.4 |
| + ERBF, 20 μM (bottom well) | 2418.5 ± 397.3 |
CSFE-labeled PMNLs (1 × 105) or 20 ng/ml of rhIL-8 were added to the top chamber or bottom chamber, respectively. ERBF (20 μM) was added to the top or bottom chamber, and 5 μg of anti-IL-8 monoclonal antibody was added to the bottom chamber. After 4-h incubation at 37°C, fluorescence intensity of the bottom chamber residue was measured by fluorometry. Values represent mean ± S.E. of three or four experiments.