Table 3

Quantitation of MHC Class II, VCAM-1, and E-selectine expression

Groups
WeekECUWICPEG50ICPEG30
MHC class II expression2
 Endothelial cells +++ + +++ +++ ++ 3-150
 Tubular cells +++ + ++ ++ + 3-150
 Inflammatory cells +++ + +++ +++ ++ 3-150
 Endothelial cells4–5 +++ +++ +++ ++ 3-150
 Tubular cells +++ ++ ++ 0/+ **
 Inflammatory cells +++ +++ +++ ++ 3-150
 Endothelial cells10–12 +++ +++ +++ ++ 3-150
 Tubular cells +++ ++ ++ 0/+ 3-150
 Inflammatory cells +++ +++ +++ ++ 3-150
VCAM-1 expression2 ++ + + 03-150
4–5 +++ ++ ++ + 3-150
10–12 +++ +++ +++ ++ 3-150
E-selectin expression2 ++ + + 03-150
4–5 ++ + + 03-150
10–12 +/++ + + 03-150

The degree of MHC class II, VCAM-1, and E-selectin staining was also determined semiquantitatively, estimated using a scale from 0 to 4+ (4+= strongest intensity), and the number of MCA1218, CD4+, and CD8+ labelled cells per surface area (104/μm2) was counted on five different tissue sections for each of the experimental conditions.

  • 3-150P < 0.05 ICPEG30 versus EC, UW, or ICPEG50.