Effect of ICI 182,780, estradiol benzoate, tamoxifen, and 4-hydroxytamoxifen on daily food intake and food efficiency
| Treatment | Daily Food Intake (mg/g body wt) and (Food Efficiency) (g wt gain/g food intake) | |||||
|---|---|---|---|---|---|---|
| Day 1 | Day 3 | Day 8 | Day 13 | Day 18 | Day 37 | |
| Propylene glycol | 74.6 ± 3.6 | 83.4 ± 0.3 | 79.3 ± 5.8 | 67.6 ± 3.6 | 67.3 ± 0.3 | 57.2 ± 1.3 |
| (0.39 ± 0.06) | (0.13 ± 0.07) | (0.09 ± 0.05) | (0.09 ± 0.08) | (0.06 ± 0.04) | (0.11 ± 0.09) | |
| ICI 182,780 | 75.0 ± 3.6 | 81.4 ± 3.4 | 72.1 ± 1.7 | 71.2 ± 3.0 | 73.1 ± 10.0 | 63.5 ± 4.2 |
| (0.3 ± 0.07) | (0.2 ± 0.01) | (0.03 ± 0.03) | (0.06 ± 0.03) | (0.05 ± 0.02) | (0.04 ± 0.01) | |
| Peanut oil | 71.8 ± 2.5 | 68.9 ± 3.2 | 71.9 ± 2.9 | 69.6 ± 0.7 | 64.7 ± 8.4 | 58.8 ± 4.2 |
| (0.06 ± 0.15) | (0.16 ± 0.02) | (0.07 ± 0.04) | (0.12 ± 0.01) | (−0.03 ± 0.06) | (−0.02 ± 0.04) | |
| Estradiol benzoate | 59.4 ± 5.9 | 53.9 ± 1.82-150 | 72.3 ± 3.7 | 72.3 ± 1.6 | 75.4 ± 1.7 | 66.6 ± 3.9 |
| (0.08 ± 0.13) | (−0.13 ± 0.07) | (0.14 ± 0.03) | (0.05 ± 0.04) | (0.08 ± 0.01) | (0.06 ± 0.05) | |
| Tamoxifen | 55.3 ± 2.22-150 | 40.0 ± 6.62-150 | 57.5 ± 3.42-150 | 61.1 ± 2.82-150 | 62.7 ± 3.4 | 63.4 ± 0.5 |
| (0.23 ± 0.1) | (−0.72 ± 0.46) | (0.04 ± 0.06) | (0.05 ± 0.06) | (0.01 ± 0.01) | (0.07 ± 0.03) | |
| Tamoxifen + Estradiol benzoate | 45.0 ± 5.42-150 | 47.5 ± 7.02-150 | 53.5 ± 2.32-150 | 64.1 ± 1.6 | 63.3 ± 0.8 | 61.4 ± 8.8 |
| (−0.17 ± 0.15) | (−0.18 ± 0.03) | (0.08 ± 0.03) | (0.08 ± 0.01) | (0.02 ± 0.04) | (0.08 ± 0.21) | |
| 4-Hydroxytamoxifen | 54.2 ± 3.42-150 | 37.0 ± 2.62-150 | 50.4 ± 2.42-150 | 61.3 ± 2.02-150 | 63.6 ± 0.6 | 56.3 ± 0.5 |
| (−0.01 ± 0.09) | (−0.69 ± 0.13) | (0.06 ± 0.02) | (0.02 ± 0.02) | (0.06 ± 0.01) | (0.00 ± 0.08) | |
Rats were injected once daily for two consecutive days with propylene glycol (2 ml/kg), ICI 182,780 (10 mg/kg), peanut oil (2 ml/kg), estradiol benzoate (1 mg/kg), tamoxifen (20 mg/kg), tamoxifen (20 mg/kg) plus estradiol benzoate (1 mg/kg), or 4-hydroxytamoxifen (20 mg/kg), and killed 37 days after the first injection. Food intake was determined by measuring the amount of food provided to each cage (two rats per cage) and the amount remaining at the next time point. Daily food intake is presented as the mean ± S.E.M. of eight rats per treatment group for days 1, 3, 8, and 13 after the first injection, and four rats per treatment group for days 17 and 37, except for the tamoxifen plus estradiol benzoate treatment group wheren = 9 and n = 5, respectively. Mean food efficiency ± S.E.M., calculated as daily weight gain (grams) divided by daily food intake (grams), is shown in parentheses. Food intake and food efficiency were also determined for each treatment group on days 23, 28, and 33, but values were not different from those listed for days 18 and 37 and thus are not included in the table.
↵2-150 Significantly different from the vehicle-treated group (p < 0.05).