Binding profile of anandamide, methanandamide, 2-AG, and oleamide to CB1 receptors in brains of FAAH(+/+) and (−/−) mice
| FAAH(+/+) | FAAH(−/−) | |
|---|---|---|
| Apparent Ki(95% C.I.) | ||
| Anandamide | 794 (300–2104) | 52 (22–127)3-160 |
| Anandamide and PMSF | 61 (17–221) | 48 (15–157) |
| Methanandamide | 75 (9–616) | 92 (22–384) |
| 2-AG | 1,890 (980–3,650) | 2,100 (1,230–3,560) |
| Oleamide (10,000 nM) | 46 ± 23% displacement | 38 ± 15% displacement |
Inhibition constants (nanomolar) were derived from displacement studies of [3H]CP55,940 at 0.5 nM (see Materials and Methods for details) (n = 3/group).
↵3-160 Significantly different from FAAH(+/+) mice (t test, p < 0.01).