NGF | Gö6976 | NGF +Gö6976 | |
---|---|---|---|
DNA synthesis | 101% ± 5.91-a | 72.6% ± 5.2 | 69.8% ± 7.0 |
Transglutaminase activity | 1.14 ± 0.301-b | 2.08 ± 0.28 | 1.96 ± 0.45 |
Near confluent primary keratinocytes were treated for 24 h with either medium + vehicle (0.1% DMSO) (control) or 100 ng/ml NGF, 1 μM Gö6976, or both. Cells were then analyzed for [3H]thymidine incorporation into DNA and transglutaminase activity as described under Materials and Methods.