Drug specificities of H/F-tagged α1-AR subtypes stably expressed in HEK293 cells
| −log KI(M) | |||||
|---|---|---|---|---|---|
| NE | Prazosin | Yohimbine | (+)-Niguldipine | BMY 7378 | |
| H/Fα1A | 5.06 ± 0.23 | 9.16 ± 0.07 | 5.50 ± 0.16 | 8.32 ± 0.12 | 6.13 ± 0.08 |
| H/Fα1B | 5.12 ± 0.15 | 9.21 ± 0.08 | 5.62 ± 0.15 | 6.35 ± 0.11 | 6.28 ± 0.07 |
| H/Fα1D | 4.98 ± 0.22 | 9.42 ± 0.12 | 6.02 ± 0.10 | 6.29 ± 0.15 | 8.60 ± 0.13 |
Membranes were collected from HEK293 cells stably expressing each tagged α1-AR subtype. Affinity constants for agonists and antagonists were determined by inhibition of specific 125I-BE (50 pM) binding, as described in the text.