Inhibition of LPS-induced cytokine synthesis in blood leukocytes by SCH 351591, SCH 365351, and cilomilast
| Compound | IC50 | ||
|---|---|---|---|
| TNFα | IL-5 | IL-12 | |
| nM | |||
| SCH 351591 | 59 (22, 157) [4] | 50 (8, 324) [6] | 14 (2, 86) [6] |
| SCH 365351 | 12 (2, 94) [3] | 215 (115, 315) [2] | N.D. |
| Cilomilast | 106 (50, 225) [18] | 170 [1] | 60 (34, 108) [5] |
Cytokines released by LPS-treated human PBMCs (TNFα and IL-12) or buffy coat cells (IL-5) were measured by ELISA and concentrations that caused 50% inhibition (IC50) were determined. Values are displayed as geometric means with 95% confidence limits in parentheses and number of experiments in brackets.
N.D., not done.