Physiological measurements during BBB/BTB permeability determination
| Groups | pH | PaO2 | PaCO2 | MAP | CBF |
|---|---|---|---|---|---|
| mm Hg | % change vs. vehicle | ||||
| Vehicle | 7.3 ± 0.02 | 87 ± 4 | 60 ± 5 | 90 ± 5 | 0 |
| BK-treated (10 μg/kg/min) | 7.38 ± 0.01 | 90 ± 5 | 54 ± 4 | 62 ± 6*** | 30** |
| NS-1619 (6.66 μg/kg/min) | 7.35 ± 0.02 | 87 ± 6 | 58 ± 4 | 81 ± 5 | N.D. |
| NS-1619 (13.33 μg/kg/min) | 7.32 ± 0.01 | 81 ± 5 | 51 ± 6 | 79 ± 6 | N.D. |
| NS-1619 (26.66 μg/kg/min) | 7.36 ± 0.02 | 89 ± 4 | 53 ± 3 | 80 ± 5 | 5 ± 2 |
| NS-1619 (39.99 μg/kg/min) | 7.38 ± 0.01 | 91 ± 3 | 50 ± 6 | 64 ± 5*** | N.D. |
| NS-1619 (59.99 μg/kg/min) | 7.38 ± 0.02 | 69 ± 6 | 61 ± 4 | 52 ± 4*** | N.D. |
| YC-1 (13.33 μg/kg/min) | 7.42 ± 0.02 | 82 ± 4 | 52 ± 4 | 73 ± 3 | 9 ± 5 |
| DEA-NONOate (26.66 μg/kg/min) | 7.36 ± 0.01 | 82 ± 3 | 56 ± 3 | 66 ± 5** | 10 ± 5 |
The physiological parameters were determined either before or during the infusion of vasomodulators. The values are mean ± S.D. ofn ≥ 4 rats, except for CBF where three representative rats per group were used. pH is arterial pH; PaO2 is arterial oxygen; PaCO2 is arterial carbon dioxide; and MAP is mean arterial blood pressure. For BTB permeability by QAR, a vasomodulator dose was selected that did not appreciably alter MAP in rats. Data of treated group were significantly different (****P < 0.001; **P < 0.01) when compared to control group.
N.D., not determined.