Sample | % Activity Remaining | % P450 Remaining | ||
---|---|---|---|---|
0 min | 20 min | 0 min | 20 min | |
−Tam, −NADPH | 100 | 93 ± 2 | 100 | 96 ± 16 |
+Tam, −NADPH | 80 ± 2 | 71 ± 4 | 102 ± 11 | 99 ± 18 |
+Tam, +NADPH | 79 ± 7 | 27 ± 4 | 103 ± 14 | 91 ± 12 |
CYP2B6 (0.5 nmol/ml) was incubated with 6.7 μM of tamoxifen in a reconstituted system at 37°C for 20 min, as described underExperimental Procedures. Aliquots were removed for analysis of residual activity or for the amount of P450 remaining measured from the reduced CO spectrum. Each data point represents the average and standard deviation from three separate experiments.