Effect of nicotine on oxygen uptake, ketogenesis, glucose production, and lactate + pyruvate production in perfused livers from fed and fasted rats
| Treatment | Oxygen Uptake | Ketone Bodies | Glucose | L + P | L/P |
|---|---|---|---|---|---|
| μmol/g/h | |||||
| Fed | |||||
| Basal | 105 ± 5 (5) | 13 ± 3 (5) | 102 ± 10 (5) | 105 ± 8 (5) | 6 ± 1 (4) |
| 85 μM Nicotine | 116 ± 6 (5) | 10 ± 2 (5) | 90 ± 10 (5) | 93 ± 8 (5)1-b | 13 ± 3 (4) |
| 850 μM Nicotine | 134 ± 8 (5)1-a | 9 ± 2 (5) | 88 ± 8 (5) | 52 ± 9 (5)1-a | 11 ± 2 (4) |
| Fasted | |||||
| Basal | 113 ± 6 (5) | 26 ± 3 (6)1-c | |||
| 85 μM Nicotine | 114 ± 7 (5) | 23 ± 4 (3) | |||
| 850 μM Nicotine | 110 ± 8 (5) | 18 ± 5 (3) |
Nicotine at concentrations indicated was infused into livers perfused with Krebs-Henseleit bicarbonate buffer saturated with oxygen/carbon dioxide mixtures (95:5) in a nonrecirculating system. Oxygen uptake was calculated from influent minus effluent oxygen concentration differences, the flow rate and tissue wet weight. Samples of effluent perfusate were collected and analyzed for metabolites as described under Materials and Methods. The cytosolic redox ratio (L/P) was calculated from lactate and pyruvate concentrations in the perfusate. Results are expressed as mean ± S.E.M., withn in parentheses.