Agonist potency for D2L receptor-mediated functions in MN9D cells
| Compound | Inhibition of Adenylate Cyclase EC50 | Inhibition of Dopamine Release EC50 |
|---|---|---|
| nM | ||
| (−)-Quinpirole | 287 ± 78 | 443 ± 99 |
| Dopamine | 264 ± 91 | N.D. |
| N-Pr-DHX | 279 ± 168 | >10 μM |
| DHX | 413 ± 184 | >10 μM |
| R-(−)-NPA | 421 ± 87 | 538 ± 63 |
| S-(+)-NPA | 1213 ± 232 | 822 ± 196 |
Data were derived from analysis of concentration-response curves obtained for each test compound for inhibition of forskolin-stimulated cAMP and K+-stimulated [3H]dopamine release in D2L receptor-transfected MN9D cells. Potency estimates were obtained by nonlinear regression of concentration-response analysis (sigmoid algorithm, InPlot; GraphPad Software). Data for each drug represent the average (mean ± S.E.M.) obtained from individual concentration-response curves (n = 3–5 for all compounds). Typical curves for selected compounds are shown in Figure3.
N.D., not determined.