Ex vivo hydrolysis of SV to SVA in dog plasma and buffer
| Sample | Animal | 0.1 μM SV | 1 μM SV | ||
|---|---|---|---|---|---|
| Period I | Period II | Period I | Period II | ||
| Plasma | Treatment group (n = 5) | 21.9 ± 1.7 | 18.7 ± 0.9 | 21.4 ± 2.7 | 18.3 ± 2.5 |
| Untreated group (n = 3) | 20.4 ± 0.4 | 16.7 ± 0.3 | 19.0 ± 0.5 | 18.1 ± 2.4 | |
| Buffer | 2.3 ± 0.4 | 2.5 ± 0.3 | 4.0 ± 0.4 | 3.5 ± 0.3 | |
Incubations were performed, in duplicate or triplicate, using plasma from dogs pretreated with vehicle (period I) or GFZ (period II) (treatment group, n = 5), and 0.1 and 1 μM SV, at 37°C for 60 min. Control hydrolysis experiments also were conducted on the same days in plasma from a different group of dogs without prior treatment (untreated group, n = 3), or in buffer. Results (percentage of SVA formed/h) are means ± S.D. (n = 3–5).