Table 3

Effects of xanomeline on rates of [³H]NMS association (K_obs) and dissociation (K_off) at M₁ receptors

Xanomeline Concentration	Simultaneous Presence of Xanomeline		Preincubation with Xanomeline
Xanomeline Concentration	K_obs	K_off	K_obs	K_off
		min
None	0.34 ± 0.01	0.044 ± 0.002	0.32 ± 0.03	0.042 ± 0.004
0.01 mM	0.33 ± 0.01
0.1 mM	0.13 ± 0.01^3-a	0.045 ± 0.002
1 mM		0.045 ± 0.003	0.33 ± 0.04	0.052 ± 0.004^3-a
10 mM			0.27 ± 0.02^*	0.102 ± 0.009^3-a

Values of K_obs and K_off were obtained by nonlinear regression analysis of data from Fig. 4(K_obs) and Fig. 5 (K_off). Data are means ± S.D. of three to six experiments.

↵3-a Significantly different from control (none),P < 0.05, two-way analysis of variance.