The effects of 17β-estradiol and raloxifene on secretory spike kinetics
| Imax | t1/2 | Q | m | tP | ncells | n spikes | |
|---|---|---|---|---|---|---|---|
| pA | ms | pC | nA/s | ms | |||
| Control | 28.7 ± 4.2 | 31.7 ± 3.0 | 0.94 ± 0.1 | 11.5 ± 2.8 | 28.8 ± 5.1 | 12 | 1115 |
| 17β-Estradiol 10 nM | 18.2 ± 2.41-150 | 47.6 ± 5.91-150 | 1.0 ± 0.1 | 4.5 ± 0.91-150 | 38.9 ± 4.9 | 12 | 927 |
| Control | 36.6 ± 6.1 | 36.7 ± 4.4 | 1.35 ± 0.1 | 9.3 ± 3.3 | 29.4 ± 4.2 | 10 | 1533 |
| Raloxifene 10 nM | 22.7 ± 2.81-150 | 54.4 ± 3.41-150 | 1.38 ± 0.1 | 2.6 ± 0.41-150 | 63.6 ± 8.41-150 | 12 | 819 |
Inset figure describes the kinetic parameters measured.Imax is the maximal current caused by the CA reaching the electrode; t1/2 is the spike width at its half height; Q is the integrated area under the spike trace that indicates the total CA released during the exocytotic event;m is the ascending slope, horizontal ticks indicate the 25 and 75% of Imax where m is calculated; tP is the time need to reach the spike maximum. Secretory spikes from cells treated 10 min with 10 nM estrogens are compared with untreated control groups. Data are expressed in the units indicated. Data were calculated by averaging spike parameters from each cell (means ± S.E.M.). The number of spikes computed was not taken into account for statistic analysis.
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↵1-150 Statistic differences (p < 0.05) from controls (Mann-Whitney tests).