Concentrations for half-maximal effects (−log EC50 values) of the muscarinic agonists pilocarpine and oxotremorine M under the indicated assay conditions.
| Assay | Agonist Concentrations (−log values) for Half-Maximal Effects | EC50,pilo/EC50,oxo | Reference | |
|---|---|---|---|---|
| Pilocarpine | Oxotremorine M | |||
| Reduction of contraction force in guinea pig left atria | 6.10 (Fig.1A) | 7.92 (Fig.2A) | 66 | This study |
| Stimulation of [35S]GTPγS binding in M2expressing CHO cells | 5.48 (Fig.4A) | 7.32 (Fig.5A) | 69 | This study |
| Inhibition of [3H]oxotremorine binding in M2 containing membranes | 6.77 (Fig.6A) | 8.82 (Fig.6A) | 112 | This study |
| Inhibition of [3H]NMS binding in M2 containing membranes in the presence of 0.5 mM GTP | 4.92 | 4.92 | 1 | Jakubı́k et al., 1997 1-a |
↵1-a Radioligand binding data taken from Jakubik et al. (1997) (membranes of CHO cells expressing human M2receptors; 136 mM NaCl, 5 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, 0.5 mM GTP, 10 mM sodium HEPES, pH 7.4, 25°C).