Effect of different adenosine receptor subtypes agonists on the parameters of the Ap5A dose-response curve
| Experimental Conditions | First Component | Second Component | ||
|---|---|---|---|---|
| EC50 | Effect of Maximal Response | EC50 | Effect of Maximal Response | |
| % | μM | % | ||
| Control (n = 3) | N.A. | N.A. | 33.5 ± 4.5 | 100 ± 8.6 |
| A1 Agonist CPA (n = 3) | 41.1 ± 1.9 pM** | 91.3 ± 5.6 | 17.1 ± 0.8 | 139.5 ± 11.2* |
| A2A Agonist CGS 21680 (n = 3) | 99.9 ± 10.2 nM** | 111.5 ± 10.5 | 21.6 ± 1.4 | 306.9 ± 28.5*** |
| A3 Agonist Cl-IB-MECA (n = 3) | N.A. | N.A. | 331.4 ± 54.6** | 98.2 ± 9.2 |
The substances were assayed under the conditions described underExperimental Procedures. The EC50 values represent the concentration of Ap5A, which is necessary to produce 50% of the maximal effect in each step. The EC50 values were statistically significant compared to control (**P < 0.005 Mann-Whitney Utest). Maximal effect values were statistically significant when compared to control (*P < 0.01 and***P < 0.001 ANOVA test), which correspond to increase on intrasynaptosomal Ca2+ of 24.4 ± 2.1 nM.n represents number of synaptosomal preparation. These results are the mean ± S.E.M. of at least three experiments performed in triplicate.
N.A., not applicable.