Effect of MCh on CaM kinase II, NOS, PKG activities, and cGMP accumulation in parotid tissue slices
| Agonist | CaM Kinase II Activity | NOS Activity | PKG Activity | cGMP |
|---|---|---|---|---|
| pmol/min/mg protein | fmol/mg protein | pmol/mg protein | fmol/mg protein | |
| None | 0.108 ± 0.010 | 63.9 ± 4.0 | 6.76 ± 0.29 | 26.7 ± 2.3 |
| MCh (1 μM) | 0.145 ± 0.0153-150 | 90.1 ± 3.53-150 | 11.95 ± 0.113-150 | 39.6 ± 2.63-150 |
Tissue slices were incubated with or without 1 μM MCh for 10 min and then assayed for CaM kinase II and NOS activities. In experiments to assay PKG activity, tissue was incubated for 10 min with or without 1 μM MCh. In the case of NOS activity, parotid tissue was prepared from rats that had been injected with l-[3H]arginine 1 h previously, as described under Materials and Methods. Data are means ± S.E. of values from three to five experiments.
↵3-150 P < 0.01 versus control value (no agonist).