Stimulant | Inhibitor (μM) | Amylase Activity |
---|---|---|
mg maltose/100 mg weight | ||
None | 48.2 ± 1.2 | |
KT5823 (10) | 48.4 ± 1.2 | |
None | KT5720 (10) | 55.8 ± 0.27 |
None | 139.6 ± 3.14-160 | |
MCh (1 μM) | KT5823 (10) | 49.5 ± 2.7 |
KT5720 (10) | 132.7 ± 8.3 | |
None | 86.8 ± 4.24-150 | |
A23187 (10 μM) | KT5823 (10) | 51.2 ± 1.9 |
KT5720 (10) | 88.9 ± 1.74-150 |
Tissue slices were pretreated for 10 min with or without the indicated concentrations of KT5823 or KT5720 and then incubated for 10 min in the additional absence or presence of MCh or A23187. The amylase activity in the incubation medium was then determined. Data are means ± S.E. of values from three experiments.