Binding Assay | Results | |
---|---|---|
CRF1 binding: [125I-Tyr0]ovine CRF ligand | pK i | n |
hCRF1-CHO cells | 8.73 ± 0.15 | 1.25 ± 0.10 |
Y 79 cells | 9.08 ± 0.20 | 0.97 ± 0.17 |
Rat brain | 8.77 ± 0.23 | 0.89 ± 0.12 |
Mouse brain | 8.90 ± 0.10 | 1.16 ± 0.13 |
Gerbil brain | 9.00 ± 0.00 | 0.75 ± 0.11 |
CRF2α binding: [125I-Tyr0] ovine sauvagine ligand | Inhibition at 10 μM | |
hCRF2α-CHO cells | 0% | |
CRF-BP binding: [125I-Tyr0] rat/human CRF ligand | Inhibition at 10 μM | |
hCRF-BP | 0% | |
rCRF-BP | 0% |
Values of pKi and Hill coefficients are the means ± S.E.M. of three experiments performed in triplicate.