Correlation of antibody consumption to liposome circulation longevity
| [Biotin]1-a | [PEG-lipid]1-b | Lipid Remaining at 1 h after Injection1-c | C20 1-d |
|---|---|---|---|
| mol% | μmol/ml plasma | mM | |
| 0 | 0 | 2.6 ± 0.21 | >25 |
| 0.1 | 0 | 0.0148 ± 0.0045 | 0.131 |
| 0.1 | 2 | 0.48 ± 0.23 | 20.3 |
| 0.1 | 5 | 2.53 ± 0.3 | >50 |
| 1 | 0 | 0.019 ± 0.004 | <0.0025 |
| 1 | 5 | 0.909 ± 0.52 | 1.5 |
↵1-a Biotinylated lipid concentration in liposomes measured in mol%.
↵1-b PEG-lipid concentration in liposomes measured in mol%.
↵1-c Plasma lipid concentration measured in mice with an established anti-biotin antibody response obtained from Figs. 2 and 3.
↵1-d Lipid concentration required to mediate 20% consumption obtained from Fig. 5.