Table 1

Effect of 10 μM H89 and 5 μM Y-27632 on formation of cell extensions induced by 10 μM forskolin

Percentage of Cells with ExtensionsPercentage of Cells with Branched ExtensionsLength of Longest Extension
μm
Co57.1  ± 2.823.4  ± 2.187.0  ± 6.1
Y-2763280.8  ± 31-a 47.3  ± 3.41-a 144.2  ± 10.01-a
H8979.9  ± 4.21-a 44.9  ± 2.21-a 140.3  ± 9.61-a
Forskolin83.9  ± 1.31-a 82.4  ± 2.41-a 99.3  ± 7.1
Forskolin + H8984.7  ± 2.11-a 60.4  ± 2.51-a 1-b 155.0  ± 10.31-a 1-b
Forskolin +Y-2763282.2  ± 2.41-a 94.9  ± 1.11-a 1-b 127.5  ± 10.01-a 1-b

Cells with extensions ≥50 μm and cells with branched extensions are shown as percentage of total number of cells analyzed. Mean ± S.E.M.; n ≥ 150. Length of longest extension is given in micrometers; n = 48.

  • 1-a  Significant difference (P < 0.05) to respective control.

  • 1-b  Significant difference (P < 0.05) to respective group treated with forskolin.