Effect of ambroxol on silica-induced nitric oxide production in macrophages
| Compound | Nitric Oxide (μM) | |
|---|---|---|
| Without Silica | With Silica | |
| Control | 2.10 ± 0.08 | 4.18 ± 0.17 |
| + Ambroxol | ||
| 5 μM | 2.32 ± 0.131-a | |
| 10 μM | 1.79 ± 0.111-a | |
| 50 μM | 1.16 ± 0.121-a | |
| 100 μM | 1.32 ± 0.091-a | 0.89 ± 0.141-a |
| + Staurosporine 100 nM | 2.16 ± 0.09 | 1.88 ± 0.211-a |
| + Genistein 10 μM | 2.08 ± 0.05 | 3.00 ± 0.171-a |
| + NMMA 500 μM | 0.45 ± 0.071-a | 1.08 ± 0.151-a |
| + L-NIL 50 μM | 0.46 ± 0.051-a | 1.59 ± 0.181-a |
| + L-NIL 100 μM | 0.38 ± 0.061-a | 1.23 ± 0.061-a |
Alveolar macrophages (3 × 105 cells) were incubated with various compounds in the presence or absence of 50 μg/ml silica for 6 h. Data represent mean ± S.E.M. of five and six replicate values in two separate experiments.
↵1-a Statistically different from intact macrophages or silica alone (P < 0.05).