Table 2

Contribution of CYP2C8 expressed in human lymphoblastoid cell lines vs. yeast to AQ N-desethylation in HLMs by RAF method

MicrosomesAQ-Desethylase Activity
CYP2C8HLM
Lymphoblastoid Cell LinesYeast
CL (measured)2.14.4608.2
RAFv, CYP2C8 268.8151.1
CL (predicted)562.3660.7
% Contribution92.5108.6

Measured CL data shown are in units of microliters per minute per milligram for HLM and microliters per minute per picomoles for CYP2C8; predicted CL data shown are in units of microliters per minute per milligram.