Band | Controls1-a | Halothane1-b | Chloroform1-b | |||||||
---|---|---|---|---|---|---|---|---|---|---|
MW (kDa)1-c | Stoich1-d | IC50 | (95% CI)1-e | Hill Slope | %D1-f | IC50 | (95% CI)1-e | Hill Slope | %D1-f | |
Membrane Protein | ||||||||||
1 | 101.5 | 4.1 | 2.0 | (0.1–77) | −0.8 | 96 | 2.2 | (0.7–7.0) | −1.8 | 46 |
2 | 54.2 | 1.6 | 0.9 | (0.5–1.6) | −1.2 | 67 | 2.0 | (1.3–3.1) | −1.9 | 46 |
3 | 48.5 | 1.2 | 0.7 | (0.5–1.0) | −1.5 | 65 | 1.8 | (0.9–3.3) | −2.0 | 44 |
4 | 45.4 | 2.2 | 0.7 | (0.4–1.2) | −1.5 | 62 | 1.7 | (0.8–3.4) | −1.8 | 43 |
5 | 37.8 | 1.4 | 0.9 | (0.1–15) | −0.8 | 75 | 2.0 | (1.3–3.2) | −1.6 | 45 |
6 | 31.9 | 1.1 | 1.1 | (0.1–26) | −0.8 | 88 | 3.0 | (2.0–4.5) | −1.4 | 62 |
7 | 29.9 | 2.1 | 0.7 | (0.3–2) | −1.1 | 60 | 2.4 | (1.2–4.7) | −2.4 | 41 |
8 | 25.7 | 2.7 | 1.2 | (0.2–9) | −1.1 | 69 | 2.9 | (0.9–9.6) | −2.0 | 35 |
9 | 21.3 | 0.2 | 0.7 | (0.2–2.8) | −0.9 | 68 | 2.9 | (2.3–3.7) | −1.4 | 49 |
10 | 18.6 | 0.1 | 0.9 | (0.3–3)1-150 | −0.51-150 | —1-150 | 10.41-150 | —1-150 | −0.51-150 | —1-150 |
11 | 17.4 | 0.2 | 1.3 | (0.0–7) | −0.5 | 99 | 14.71-150 | —1-150 | −0.51-150 | —1-150 |
12 | 15.0 | 0.1 | 0.8 | (0.1–21) | −0.5 | 88 | 2.4 | (1.5–3.7) | −0.9 | 63 |
Soluble Protein | ||||||||||
1 | 67.2 | 3.7 | 0.6 | (0.4–0.9) | −1.1 | 70 | 0.6 | (0.4–0.9) | −1.0 | 73 |
2 | 51.6 | 3.2 | 0.6 | (0.2–1.9) | −0.9 | 71 | 0.7 | (0.5–1.2) | −1.2 | 66 |
3 | 43.5 | 2.2 | 0.4 | (0.3–0.5) | −0.9 | 74 | 0.6 | (0.3–1.3) | −1.1 | 71 |
4 | 37.2 | 1.4 | 0.5 | (0.3–0.8) | −0.8 | 77 | 0.6 | (0.3–1.4) | −0.9 | 74 |
5 | 31.1 | 1.6 | 0.6 | (0.3–0.9) | −1.0 | 69 | 0.7 | (0.2–2.1) | −0.9 | 70 |
6 | 29.6 | 2.4 | 0.7 | (0.3–1.5) | −0.9 | 71 | 0.7 | (0.3–1.7) | −0.8 | 70 |
7 | 26.3 | 1.7 | 0.5 | (0.4–0.5) | −1.0 | 74 | 0.8 | (0.5–1.3) | −0.8 | 78 |
8 | 21.3 | 0.7 | 0.4 | (0.4–0.5) | −1.1 | 70 | 0.8 | (0.5–1.1) | −0.7 | 83 |
9 | 19.0 | 0.3 | 0.4 | (0.2–0.6) | −1.0 | 72 | 0.9 | (0.7–1.1) | −0.7 | 82 |
10 | 15.8 | 0.6 | 0.3 | (0.2–0.5) | −0.9 | 73 | 0.9 | (0.7–1.3) | −1.1 | 72 |
11 | 13.5 | 1.5 | 0.6 | (0.2–2.0) | −0.9 | 75 | 1.3 | (0.5–3.5) | −1.3 | 71 |
12 | 12.4 | 0.9 | 0.2 | (0.1–0.6) | −0.9 | 68 | 0.9 | (0.3–2.9) | −1.2 | 70 |
↵1-a n = 30.
↵1-b Non-linear regressionR 2 values were 1.0 for both halothane and chloroform.
↵1-c Molecular weight.
↵1-d Stoichiometry, this is not a maximal stoichiometry (B max) but rather the apparent stoichiometry of labeling at 0.2 mM halothane.
↵1-e IC50 95% confidence interval.
↵1-f Percent displacement.
↵1-150 Indicates that in the nonlinear regression analysis the bottom values were negative and were therefore set to zero. Likewise, isoflurane was not included in the table because the reliable calculation of competition parameters could not be determined.